Nutrients sampling

The Mediterranean Sea in general, and the Calvi Bay in particular, are very oligotrophic areas. As a result, organisms living in their waters are adapted to low nutrient concentrations, and even small changes in these concentrations can cause big perturbations of natural communities.

Thanks to the mesocosm experiment, we (Sylvie Gobert from University of Liège, and Loïc Michel, from the Stareso research station) will try to understand if ocean acidification could modify nutrient concentrations in Calvi Bay. To do so, we take daily samples to monitor nitrates and nitrites, ammonium, phosphates and silicates concentrations in each of the mesocosms, as well as an “extra” sample out of the mesocosms. We look forward to seeing which trends emerge from the data, and we hope that they can be useful for other scientists taking part of the experiment too…

Once the water is sampled, we have to condition the samples. This is the most critical part of our jobs, because by this time, it is usually around 11 AM. Since labs are quite crowded, we work directly on the Stareso dock, under the burning Corsican sun. During this dangerous task, the only things that prevent us from baking are 1) our beloved straw hats (see fig. 1) and 2) a very good hydration plan based on refreshing Corsican beer. After conditioning, we place the samples in the freezer (for NO2- + NO3-, NH4+ and PO43-) or in the fridge (for SiO44-).

Nutrient conditioners hard at work under the Corsican sun.

Now the experiment is nearly finished, and nearly all samples are stored, patiently awaiting analysis. On Sunday (July 15th), our colleague Renzo Biondo (also from Lab of Oceanology, University of Liège) will join us, and we will start the analysis step. All nutrient concentrations will be determined at Stareso, using our Skalar automated continuous flow automated analyser. Methods differ for each compound, but all are based on colorimetric detection. When everything runs smoothly, this type of analysis is rather quick, and we hope to be done in about a week… However, the analyser is a whimsical machine, and a lot of things can go wrong. To ensure that the Nutrient God is with us, we consider sacrificing one of the station’s cats to him. Let’s hope it will be enough to please him!

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